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Assay Kits
A Guide to Choosing the Right ELISA Kit
ELISA Kit
G-LISA Bundle (BK135 = BK124-S + BK127-S + BK128-S)
G-LISA Bundle (BK135 = BK124-S + BK127-S + BK128-S) from Cytoskeleton, Inc.
Be the first to write a review!
Citations:
(16)
Supplier Page
Supplier Page from
Cytoskeleton, Inc. for
G-LISA Bundle (BK135 = BK124-S + BK127-S + BK128-S)
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Product Specs
Company Info
Citations
Reviews
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Product Specs
Item
G-LISA Bundle (BK135 = BK124-S + BK127-S + BK128-S)
Company
Cytoskeleton, Inc.
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
BK135
Quantity
3 KITS (24 assays/kit)
Applications
ELISA
Detection Target
RhoA
Brand/Trademark
Cytoskeleton, Inc
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Cytoskeleton, Inc.
1830 S. Acoma St.
Denver
,
Colorado
80223
United States
Phone
: 303 322-2254
303 322-2257
Company Profile
Website:
http://cytoskeleton.com/
Citations (16)
(1)
Alveolar repair following LPS-induced injury requires cell-ECM interactions
JCI insight
July 24, 2023
Sucre JM, Bock F, Negretti NM, Benjamin JT, Gulleman PM, Dong X, Ferguson KT, Jetter CS, Han W, Liu Y,
et al
.
incubation
. Cell lysates were then used for G-LISA small GTPase activation
assay
(
Cytoskeleton
catalog
BK135
), where levels of activated RhoA, Cdc42, and Rac1 were detected colorimetrically per manufacturer’s
(2)
Proteolytic regulation of a galectin-3/Lrp1 axis controls osteoclast-mediated bone resorption
The Journal of cell biology
April 3, 2023
Zhu L, Tang Y, Li XY, Kerk SA, Lyssiotis CA, Sun X, Wang Z, Cho JS, Ma J, Weiss SJ.
lysates were harvested, and Rho GTPase activity analyzed using RhoA/Rac1 G-LISA Activation
Assay
kits (
BK135
;
Cytoskeleton
) according to the manufacturer’s instructions. Lentiviral or retroviral gene transduction Where
(3)
Different hotspot p53 mutants exert distinct phenotypes and predict outcome of colorectal cancer patients
Nature Communications
December 1, 2022
Ori Hassin et al.
determined by using an
enzyme-linked immunosorbent assay
(
ELISA
)-based G-LISA kit (
Cytoskeleton, Inc
#
BK135
) strictly following the manufacturer’s instructions. Briefly, SW480 cells stably overexpressing p53R175H
(4)
Aberrant activation of TGF-β1 induces high bone turnover via Rho GTPases-mediated cytoskeletal remodeling in Camurati-Engelmann disease
Front Endocrinol (Lausanne)
October 17, 2022
Qi Chen, Yan Yao, Kun Chen, Xihui Chen, Bowen Li, Rui Li, Lidangzhi Mo, Weihong Hu, Mengjie Zhang, Zhen Wang,
et al.
respectively. Rho GTPase activation
assay
The
assay
was implemented through a GTPase G-LISA activation
assay
kit (
BK135
,
Cytoskeleton, Inc
.). After washing with PBS, RAW264.7 or HEK293T cells were treated with a cold lysis
(5)
Nucleoporin-93 reveals a common feature of aggressive breast cancers: robust nucleocytoplasmic transport of transcription factors
Cell reports
February 22, 2022
Nataraj NB, Noronha A, Lee JS, Ghosh S, Mohan Raju HR, Sekar A, Zuckerman B, Lindzen M, Tarcitano E, Srivastava S,
et al
.
LTX and Plus,Invitrogen,Cat# 15338100 Rho/Rac/cdc42 G-LISA activation kit,"
Cytoskeleton, Inc
",Cat#
BK135
3D
assay
kit,Cultrex,Cat# 3500096K FluoroBlok,
Corning
,Cat#
351157
Ras activation kit,"
Cytoskeleton, Inc
(6)
Profilin2 regulates actin rod assembly in neuronal cells
Scientific Reports
December 1, 2021
Lisa Marie Walter, Sebastian Rademacher, Andreas Pich, Peter Claus
activity of small G-proteins (Rac1, RhoA and Cdc42) was measured by utilizing G-LISA Activation
Assays
(
BK135
,
Cytoskeleton
, Denver, Colorado, USA). The
assays
were performed according to the manufacturer’s instructions.
(7)
Cancer-associated mutations in VAV1 trigger variegated signaling outputs and T-cell lymphomagenesis
The EMBO journal
November 15, 2021
Robles-Valero J, Fernández-Nevado L, Lorenzo-Martín LF, Cuadrado M, Fernández-Pisonero I, Rodríguez-Fdez S, Astorga-Simón EN, Abad A, Caloto R, Bustelo XR.
extracts were analyzed using G‐LISA
Assay
Kits according to the manufacturer’s instructions (Cat. No.
BK135
,
Cytoskeleton
). Values are represented as the n‐fold change in the experimental sample relative to the
(8)
PD-L1 recruits phospholipase C and enhances tumorigenicity of lung tumors harboring mutant forms of EGFR
Cell reports
May 25, 2021
Ghosh S, Nataraj NB, Noronha A, Patkar S, Sekar A, Mukherjee S, Winograd-Katz S, Kramarski L, Verma A, Lindzen M,
et al
.
LTX and Plus,Invitrogen,Cat# 15338100 Rho/Rac/cdc42 G-LISA activation kit,"
Cytoskeleton, Inc
",Cat#
BK135
3D
assay
kit,Cultrex,Cat# 3500096K FluoroBlok,
Corning
,Cat#
351157
Luciferase kit,Promega,Cat#
E1910
RNA
(9)
RhoG deficiency abrogates cytotoxicity of human lymphocytes and causes hemophagocytic lymphohistiocytosis
Blood
April 15, 2021
Artem Kalinichenko et al.
(quantification of GTP-bound protein) of Rac1, Cdc42, and RhoA was assessed using G-LISA Kit (
Cytoskeleton Inc
,
BK135
) according to manufacturer’s instructions. Alternatively, analysis of Rac1, Cdc42, and RhoA activation
(10)
VAV2 signaling promotes regenerative proliferation in both cutaneous and head and neck squamous cell carcinoma
Nature communications
September 22, 2020
Lorenzo-Martín LF, Fernández-Parejo N, Menacho-Márquez M, Rodríguez-Fdez S, Robles-Valero J, Zumalave S, Fabbiano S, Pascual G, García-Pedrero JM, Abad A,
et al
.
and Cdc42 G-LISA
assay
kits according to the manufacturer’s instructions (
Cytoskeleton
, Catalog No.
BK135
).
Microarray
experiments Total RNA from the back
epidermis
of 8-week-old
mice
of indicated genotypes
(11)
Vav2 pharmaco-mimetic mice reveal the therapeutic value and caveats of the catalytic inactivation of a Rho exchange factor
Oncogene
July 9, 2020
L. Francisco Lorenzo-Martín, Sonia Rodríguez-Fdez, Salvatore Fabbiano, Antonio Abad, María C. García-Macías, Mercedes Dosil, Myriam Cuadrado, Javier Robles-Valero, Xosé R. Bustelo
analyzed using G-LISA
assay
kits according to the manufacturer’s instructions (
Cytoskeleton
, Catalog No.
BK135
). Values are represented as the n-fold change of the experimental sample relative to the activity shown
(12)
The Inhibition of Wnt Restrain KRASG12V-Driven Metastasis in Non-Small-Cell Lung Cancer
Cancers (Basel)
March 31, 2020
Pei-Shan Hung, Ming-Hung Huang, Yuan-Yeh Kuo, James Chih-Hsin Yang
Assay
The activity of RhoA/Rac1/Cdc42 was determined using a RhoA/Rac1/
Cdc42 G-LISA Activation Assay kit
(
BK135
) as recommended by the manufacturer (
Cytoskeleton, Inc
., Denver, CO, USA). Briefly, the samples were
(13)
Lysine Acetylation Reshapes the Downstream Signaling Landscape of Vav1 in Lymphocytes
Cells
March 4, 2020
Sonia Rodríguez-Fdez, Lucía Fernández-Nevado, L. Francisco Lorenzo-Martín, Xosé R. Bustelo
and analyzed using Rac1 G-LISA®
assay
kits according to the manufacturer’s instructions (Cat. number
BK135
,
Cytoskeleton
). 2.11. Statistical Analyses All the statistical analyses were carried out using GraphPad
(14)
AIF1L regulates actomyosin contractility and filopodial extensions in human podocytes
PloS one
January 1, 2018
Yasuda-Yamahara M, Rogg M, Yamahara K, Maier JI, Huber TB, Schell C.
activity was measured using a
Cdc42 G-LISA activation assay kit
according to manufacturer´s instructions (
BK135
,
Cytoskeleton
, USA). AIF1L
immunoprecipitation
and interaction proteomics SILAC
labeling
and quantitative
(15)
MicroRNA-141 suppresses prostate cancer stem cells and metastasis by targeting a cohort of pro-metastasis genes
Nature Communications
April 1, 2017
Can Liu et al.
activation
assays
as per the manufacturer's instructions (
Cytoskeleton, Inc
., Denver, CO; catalogue number
BK135
).
RNA-Seq
and bioinformatics DU145 and LAPC9 cells were
transfected
with 30 nM of miR-141 or NC oligos
(16)
Cell-Intrinsic Adaptation Arising from Chronic Ablation of a Key Rho GTPase Regulator
Developmental Cell
August 30, 2016
Berati Cerikan, Ranad Shaheen, Georgina P. Colo, Christine Gläßer, Shoji Hata, Klaus-Peter Knobeloch, Fowzan S. Alkuraya, Reinhard Fässler, Elmar Schiebel
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Reviews
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Bench Tips
Bench Tips for Optimizing ELISA: It All Comes Out in the Wash
August 22, 2013
This article will provide you with tips on washing your ELISA plates using an automated plate washer, with the goal of avoiding high background and high variation (between plates and between replicate wells).
read more
G-LISA Bundle (BK135 = BK124-S + BK127-S + BK128-S) from Cytoskeleton, Inc.
Supplier Page
View Company Product Page
Pricing Info
Product Specs
Company Info
Citations
Reviews
Bench Tips
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